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Gold-Standard

Currently, the “gold-standard method” for the detection of SARS-CoV-2 is quantitative reverse transcription polymerase chain reaction (qRT-PCR). The qRT-PCR test requires significant specialized equipment, trained operators, and several hours to perform.

ULTSafety’s Freedom Chip bypasses traditional qRT-PCR diagnostics for a significantly faster, easier, cheaper assay. Unlike traditional PCR assays requiring expensive equipment, the Freedom Chip requires only a simple test reader.

RT-Lamp

ULTSafety’s assay utilizes isothermal PCR using RT-LAMP, or reverse transcriptase loop-mediated isothermal amplification. LAMP is an isothermal technique for the exponential amplification of RNA. RT-LAMP removes the bottleneck of thermal cycling and high temperatures required by regular PCR-based methods and is therefore much faster compared to qRT-PCR. At the clinical laboratory test scale, the qRT-PCR test is considered a complex test where results can only be obtained by trained personnel and can take up to 4 hours to complete. By replacing PCR with LAMP chemistry, significant gains in turnaround time are achieved.

LAMP in Drops Makes Detection Even Faster - ULTSafety’s Freedom Chip encapsulates the virus and reagents into millions of tiny drops in seconds. The smaller the drops, the faster the reaction products are concentrated, and the faster the virus can be detected.